Application
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K4me3 antibody.Application
Dot-blot analysis of all sorts of methylation peptides using H3K4me3 antibody.Application
Immunohistochemistry of paraffin-embedded rat brain tissue using H3K4me3 antibody at dilution of 1:200 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human rectal cancer tissue using H3K4me3 antibody at dilution of 1:200 (x400 lens).Application
Immunofluorescence analysis of 293T cell using H3K4me3 antibody. Blue: DAPI for nuclear staining.Application
Chromatin Immunoprecipitation analysis extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Product Name | Histone H3K4me3 (tri-methyl Lys4) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3K4me3 (tri-methyl Lys4)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3 |
| Modification | tri-methyl Lys4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHCIHC-PIPWBChIP |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP assay: 1:20 - 1:100, CHIPseq: 1:20 - 1:100: |
|
| Species Reactivity | HumanMouseRatOther |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H3.4 histone cluster 3 H3 H3FT H3/g H3t HIST3H3 |
|---|---|
| Target | Histone H3K4me3 (tri-methyl Lys4) |
Application
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K4me3 antibody.
Application
Dot-blot analysis of all sorts of methylation peptides using H3K4me3 antibody.
Application
Immunohistochemistry of paraffin-embedded rat brain tissue using H3K4me3 antibody at dilution of 1:200 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human rectal cancer tissue using H3K4me3 antibody at dilution of 1:200 (x400 lens).
Application
Immunofluorescence analysis of 293T cell using H3K4me3 antibody. Blue: DAPI for nuclear staining.
Application
Chromatin Immunoprecipitation analysis extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP assay: 1:20 - 1:100, CHIPseq: 1:20 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
