Application
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R2me2s antibody.Application
Dot-blot analysis of all sorts of methylation peptides using H3R2me2s antibody.Application
Immunofluorescence analysis of 293T cell using H3R2me2s antibody. Blue: DAPI for nuclear staining.| Product Name | Histone H3R2me2 (Symmetric dimethyl Arg2) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3R2me2 (Symmetric dimethyl Arg2)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3 |
| Modification | Symmetric dimethyl Arg2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHC-PIPWBChIP |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:20 - 1:100, CHIPseq: 1:20 - 1:100: |
|
| Species Reactivity | HumanMouseRatOther |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | histone cluster 3 H3 HIST3H3 H3FT H3t H3.4 H3/g |
|---|---|
| Target | Histone H3R2me2 (Symmetric dimethyl Arg2) |
Application
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R2me2s antibody.
Application
Dot-blot analysis of all sorts of methylation peptides using H3R2me2s antibody.
Application
Immunofluorescence analysis of 293T cell using H3R2me2s antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP: 1:20 - 1:100, CHIPseq: 1:20 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
