Application
Dot-blot analysis of all sorts of methylation peptides using Histone H3R17me1 (mono-methyl Arg17) antibody.Application
WB analysis of HeLa lysate and H3 protein using Histone H3R17me1 (mono-methyl Arg17) antibody.Application
ICC/IF analysis of 293T cells using Histone H3R17me1 (mono-methyl Arg17) antibody (green) and DAPI (blue).| Product Name | Histone H3R17me1 (mono-methyl Arg17) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3R17me1 (mono-methyl Arg17)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic mono-methylated peptide corresponding to residues surrounding Arg17 of human histone H3 |
| Modification | mono-methyl Arg17 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHC-PIPWBChIP |
| WB: 1:500 - 1:200. IHC: 1:50 - 1:20. IF: 1:50 - 1:20. IP: 1:50 - 1:20. ChIP: 1:20 - 1:10. CHIPseq: 1:20 - 1:100: |
|
| Species Reactivity | HumanMouseOther |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H3/j histone cluster 1 H3j HIST1H3J H3FJ |
|---|---|
| Target | Histone H3R17me1 (mono-methyl Arg17) |
| Molecular Weight(MW) | 15 kDa (note) |
Application
Dot-blot analysis of all sorts of methylation peptides using Histone H3R17me1 (mono-methyl Arg17) antibody.
Application
WB analysis of HeLa lysate and H3 protein using Histone H3R17me1 (mono-methyl Arg17) antibody.
Application
ICC/IF analysis of 293T cells using Histone H3R17me1 (mono-methyl Arg17) antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:200. IHC: 1:50 - 1:20. IF: 1:50 - 1:20. IP: 1:50 - 1:20. ChIP: 1:20 - 1:10. CHIPseq: 1:20 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
