Application
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R17me2a antibody.Application
Dot-blot analysis of all sorts of methylation peptides using H3R17me2a antibody.Application
Immunohistochemistry of paraffin-embedded rat lung tissue using H3R17me2a antibody at dilution of 1:200 (x400 lens)Application
Immunohistochemistry of paraffin-embedded human lung cancer tissue using H3R17me2a antibody at dilution of 1:200 (x400 lens)Application
Immunohistochemistry of paraffin-embedded human kidney cancer tissue using H3R17me2a antibody at dilution of 1:200 (x400 lens)Application
Immunohistochemistry of paraffin-embedded mouse lung tissue using H3R17me2a antibody at dilution of 1:200 (x400 lens)Application
Immunofluorescence analysis of 293T cell using H3R17me2a antibody. Blue: DAPI for nuclear staining.| Product Name | Histone H3R17me2 (di-methyl Arg17) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3R17me2 (di-methyl Arg17)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding Arg17 of human histone H3 |
| Modification | di-methyl Arg17 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHCIHC-PIPWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRatOther |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H3FJ H3/j HIST1H3J histone cluster 1 H3j |
|---|---|
| Target | Histone H3R17me2 (di-methyl Arg17) |
Application
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R17me2a antibody.
Application
Dot-blot analysis of all sorts of methylation peptides using H3R17me2a antibody.
Application
Immunohistochemistry of paraffin-embedded rat lung tissue using H3R17me2a antibody at dilution of 1:200 (x400 lens)
Application
Immunohistochemistry of paraffin-embedded human lung cancer tissue using H3R17me2a antibody at dilution of 1:200 (x400 lens)
Application
Immunohistochemistry of paraffin-embedded human kidney cancer tissue using H3R17me2a antibody at dilution of 1:200 (x400 lens)
Application
Immunohistochemistry of paraffin-embedded mouse lung tissue using H3R17me2a antibody at dilution of 1:200 (x400 lens)
Application
Immunofluorescence analysis of 293T cell using H3R17me2a antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
