Application
Immunofluorescence analysis of U2OS cell using Phospho-HIST1H3B-T11 antibody. Blue: DAPI for nuclear staining.| Product Name | Histone H3T12ph (phospho Thr12) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3T12ph (phospho Thr12)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding T11 of human HIST1H3B |
| Modification | p-Thr12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB 1:500 - 1:2000, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H3FL HIST1H3B H3/l histone cluster 1 H3b |
|---|---|
| Target | Histone H3T12ph (phospho Thr12) |
| Molecular Weight(MW) | 15 kDa (note) |
Application
Immunofluorescence analysis of U2OS cell using Phospho-HIST1H3B-T11 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
