Application
Dotblot analysis of anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody with peptide samples. Peptide samples (0.1 μg) were spotted onto positively charged nylon membrane and blotted with Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody at different dilution as indicated. A: Peptide samples of Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) B: Peptide samples of Histone H4Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 15% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A: PC-12 15% SDS PAGE diluted at 1:10000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:10000Application
Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody detects Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein stained by Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.| Product Name | Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H4 (acetyl Lys5, Lys6, Lys12, Lys16) (histone cluster 1, H4a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq] |
| Modification | acetyl Lys5/Lys8/Lys12/Lys16 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevisXenopus laevis (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | histone cluster 1 antibody acetyl Histone H4 antibody H4/a antibody Histone4 acetyl antibody HIST1H4B antibody HIST2H4B antibody member A antibody HIST1H4E antibody histone H4 antibody HIST4H4 antibody HIST1H4H antibody HIST1H4K antibody H4a an |
|---|---|
| Molecular Weight(MW) | 11 kDa (note) |
| Cellular Localization | Nucleus |
Application
Dotblot analysis of anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody with peptide samples. Peptide samples (0.1 μg) were spotted onto positively charged nylon membrane and blotted with Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody at different dilution as indicated. A: Peptide samples of Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) B: Peptide samples of Histone H4
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 15% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A: PC-12 15% SDS PAGE diluted at 1:10000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:10000
Application
Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody detects Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein stained by Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.| Positive Control | HeLa , NIH-3T3 , BCL-1 , PC-12 , *TGW |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
