Application
Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: H1299 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using hnRNP K antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using hnRNP K antibody at 1:100 dilution.Application
hnRNP K antibody detects hnRNP K protein at nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. hnRNP K antibody dilution: 1:500.Application
hnRNP K antibody detects hnRNP K protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. hnRNP K antibody dilution: 1:500.Application
hnRNP K antibody detects hnRNP K protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. hnRNP K antibody dilution: 1:500.| Product Name | hnRNP K antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to hnRNP K (heterogeneous nuclear ribonucleoprotein K)This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human hnRNP K. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Rabbit, Rhesus Monkey, BovineXenopus laevis (81%), Dog (98%), Pig (99%), Rabbit (99%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CSBP antibody FLJ41122 antibody HNRPK antibody TUNP antibody HNRNPK antibody dC-stretch binding protein antibody transformation upregulated nuclear protein antibody heterogeneous nuclear ribonucleoprotein K antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) (6) |
| Cellular Localization | Cytoplasm , Nucleus , nucleoplasm |
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: H1299 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using hnRNP K antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using hnRNP K antibody at 1:100 dilution.
Application
hnRNP K antibody detects hnRNP K protein at nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. hnRNP K antibody dilution: 1:500.
Application
hnRNP K antibody detects hnRNP K protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. hnRNP K antibody dilution: 1:500.
Application
hnRNP K antibody detects hnRNP K protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. hnRNP K antibody dilution: 1:500.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
