Application
p38 antibody [N1C3-2] detects p38 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. p38 antibody [N1C3-2] diluted at 1:250.Application
p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. p38 MAPK antibody [N1C3-2] diluted at 1:500.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | p38 MAPK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p38 (mitogen-activated protein kinase 14)The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq] |
| Immunogen | Full length human p38 MAPK Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Sheep, Chimpanzee, BovineXenopus laevis (85%), Dog (95%), Sheep (96%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CSBP antibody CSBP1 antibody CSBP2 antibody CSPB1 antibody EXIP antibody Mxi2 antibody PRKM14 antibody PRKM15 antibody RK antibody SAPK2A antibody p38 antibody p38ALPHA antibody MAPK14 antibody mitogen-activated protein kinase 14 antibody Csa |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (2) |
| Cellular Localization | Cytoplasm (By similarity) , Nucleus |
Application
p38 antibody [N1C3-2] detects p38 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. p38 antibody [N1C3-2] diluted at 1:250.
Application
p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. p38 MAPK antibody [N1C3-2] diluted at 1:500.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T , A431 , HeLa , HepG2 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
