Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP K antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000| Product Name | hnRNP K antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to hnRNP K (heterogeneous nuclear ribonucleoprotein K)This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human hnRNP K. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rabbit, Rhesus Monkey, BovineMouse (100%), Rat (100%), Dog (100%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CSBP antibody FLJ41122 antibody HNRPK antibody TUNP antibody HNRNPK antibody dC-stretch binding protein antibody transformation upregulated nuclear protein antibody heterogeneous nuclear ribonucleoprotein K antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) (6) |
| Cellular Localization | Cytoplasm , Nucleus , nucleoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP K antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
