Application
Western Blot: hnRNP M1-M4 Antibody (1D8) - Analysis of hnRNP M1-M4 in HeLa cell lysates using| Product Name | HNRNP M1-M4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1D8] to hnRNP M1 - M4Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogenous nuclear ribonucleoproteins (hnRNPs), play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64kDa subunit of cleavage stimulation factor, which is involved in 3'-end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast. |
| Immunogen | M19 fusion protein containing full-lengthhuman protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1D8 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| For WB: Use at a dilution of 1:500 (ECL). A 64-68 kDa band can be seen in a Western blot analysis, using HeLa cell lysate. For IP: Use at a dilution of 3:25 (Protein A). Optimal dilutions/concentrations should be determined by the researcher.: |
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| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HNRNP M1 M4 HNRNP M1M4 |
|---|---|
| Target | HNRNP M1-M4 |
| Tissue Specificity | This antibody is specific for human RNP M1-M4. |
| Light Chain Type | kappa |
Application
Western Blot: hnRNP M1-M4 Antibody (1D8) - Analysis of hnRNP M1-M4 in HeLa cell lysates using| Application Notes | For WB: Use at a dilution of 1:500 (ECL). A 64-68 kDa band can be seen in a Western blot analysis, using HeLa cell lysate. For IP: Use at a dilution of 3:25 (Protein A). Optimal dilutions/concentrations should be determined by the researcher.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and Store at -70 °C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Ascites |
