| Product Name | HNRNP M1-M4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3C181] to hnRNP M1 - M4Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogenous nuclear ribonucleoproteins (hnRNPs), play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64 kDa subunit of cleavage stimulation factor, which is involved in 3-end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast. |
| Immunogen | M19 fusion protein containing full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3C181 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Use at a dilution of 3/25. WB: Use at a dilution of 1/500. Detects a band at 64-68kD using HeLa cell lysate. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMousePigRatBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HNRNP M1 M4 HNRNP M1M4 |
|---|---|
| Target | HNRNP M1-M4 |
| Tissue Specificity | Specific for human RNP M1-M4. |
| Light Chain Type | kappa |
| Application Notes | IP: Use at a dilution of 3/25. WB: Use at a dilution of 1/500. Detects a band at 64-68kD using HeLa cell lysate. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 °C.Aliquots are stable for 24 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilu |
| Storage Buffer | Ascitic fluid with sodium azide |
