Application
HEK293T cells transfected with either RC223612 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HP antibody, and then analyzed by flow cytometry.Application
Anti-HP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HPApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HP.| Product Name | HP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2B8] to HPHaptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. |
| Immunogen | Full length human recombinant protein of human HP (NP_005134) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2B8 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | hp2alpha hp2-alpha MGC111141 HP hp2 alpha Haptoglobin |
|---|---|
| Target | HP |
| Tissue Specificity | is specific for human HP (NP_005134). |
Application
HEK293T cells transfected with either RC223612 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HP antibody, and then analyzed by flow cytometry.
Application
Anti-HP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HP
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HP.| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
