Application
Cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp60 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Application
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)| Product Name | HSP60 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2E4 ] to HSP60Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E.coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding. |
| Immunogen | Recombinant human Hsp60 (1-573aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2E4 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| Western blot is 1:1,000 ~ 1:2,000 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | fa04a05 Spastic paraplegia 13 (autosomal dominant) wu:fa04a05 Spastic paraplegia 13 Mitochondrial matrix protein P1 HuCHA60 Spastic paraplegia13 Mitochondrial matrix protein P1 HSPD1 Heat shock60kD protein1 (chaperon E coli cb863 Chaperonin 60- |
|---|---|
| Target | HSP60 |
| Light Chain Type | kappa |
Application
Cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp60 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Application
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)| Application Notes | Western blot is 1:1,000 ~ 1:2,000 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
