Application
Western blot analysis of MBH90B reactivity with various cell lines and recombinant Hsp90 alpha and Hsp90 beta protein .Application
Western blotting analysis of Hsp90 alpha and beta protein by antibody MBH90AB to both Hsp90 alpha and beta isoform.| Product Name | Hsp90 alpha,beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [MBH90AB] to Hsp90 alpha,betaHsp90 (heat shock protein 90) is one of the most abundant chaperones in the cytosol of eukaryotic cells. It interacts with various proteins, including protein kinases and transcription factors, and either facilitates their stabilization and activation or directs them for proteasomal degradation. Hsp90 thus affects multiple signaling pathways and biological processes and modulation of this single target offers the prospect of simultaneous intervence to various key points of oncogenic transformation. Hsp90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis. There are two isoforms, alpha and beta, of vertebrate Hsp90. Whereas Hsp90 beta is expressed constitutively to a high level, Hsp90 alpha is stress-inducible and is overexpressed in many cancerous cells.Entrez Gene (HSP90A, human): 14q32.33Entrez Gene (HSP90B, human): 6p12 |
| Immunogen | Peptide corresponding to the sequence EEVHHGEEEVEC within N-terminal part of human Hsp90. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MBH90AB |
| Host Species | Mouse |
| Tested Applications | IPWB |
| IP: Use at 1 µg/mL: |
|
| Species Reactivity | HumanMouseBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | D6S182 HSP90-BETA HSP90B HSPC2 |
|---|---|
| Target | Hsp90 alpha,beta |
| Tissue Specificity | The antibody MBH90AB recognizes the epitope EEEVE within N-terminal part of ubiquitously expressed Hsp90 alpha and Hsp90 beta proteins with calculated Mw of 84.7 kDa and 83.3 kDa, respectively, however, migrating as 90 kDa bands under reducing SDS-PAGE conditions. |
| Cellular Localization | Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Application
Western blot analysis of MBH90B reactivity with various cell lines and recombinant Hsp90 alpha and Hsp90 beta protein .
Application
Western blotting analysis of Hsp90 alpha and beta protein by antibody MBH90AB to both Hsp90 alpha and beta isoform.| Application Notes | IP: Use at 1 µg/mL: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
