Application
Western blotting analysis ofBH90B reactivity with various cell lines and recombinant Hsp90 alpha and Hsp90 beta protein.?| Product Name | Hsp90 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [MBH90B] to Hsp90 betaHsp90 beta (heat shock protein 90 beta) is a constitutively expressed isoform of Hsp90, one of the most abundant chaperones in the cytosol of eukaryotic cells. Hsp90 interacts with various proteins, including protein kinases and transcription factors, and either facilitates their stabilization and activation or directs them for proteasomal degradation. Hsp90 thus affects multiple signaling pathways and biological processes and modulation of this single target offers the prospect of simultaneous intervence to various key points of oncogenic transformation. Hsp90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis.Entrez Gene (HSP90B, human): 6p12 |
| Immunogen | Peptide corresponding to the EEVHHGEEEVEC sequence within N-terminal part of human Hsp90. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MBH90B |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| IP: Use at 1 µg/mL: |
|
| Species Reactivity | HumanMouseBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | D6S182 HSP90-BETA HSP90B HSPC2 |
|---|---|
| Target | Hsp90 beta |
| Tissue Specificity | The antibody MBH90B recognizes the EEVHHG epitope within the N-terminal part of Hsp90 beta an ubiquitously expressed protein with calculated Mw of 83.3 kDa, however, migrating as a 90 kDa band under reducing SDS-PAGE conditions. |
| Cellular Localization | Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Application
Western blotting analysis ofBH90B reactivity with various cell lines and recombinant Hsp90 alpha and Hsp90 beta protein.?| Application Notes | IP: Use at 1 µg/mL: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
