| Product Name | Human lambda Light chain antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2G9] to Lambda Light chainAll five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). |
| Immunogen | Mixture of light chains of human immunoglobulin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2G9 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FIHC-P |
| ELISA: Use at an assay dependent dilution. Specific antibody titer is 1/100000 by indirect ELISAIHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | IGLV214 V1-4 |
|---|---|
| Target | lambda Light chain |
| Tissue Specificity | Specific for independent constantly expressed epitope of lambda chain. Reacts with free and heavy chain-associated lambda chains. |
| Application Notes | ELISA: Use at an assay dependent dilution. Specific antibody titer is 1/100000 by indirect ELISAIHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4 |
