Application
IHC Image using - Detection of Lambda Light Chain by IHC in Human Tonsil| Product Name | Human lambda Light chain antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Mouse Antibody to Human Lambda Light ChainThis antibody is specific to human lambda light chain. It reacts with free and bound heavy chain isotypes. Lambda light chains are found on the surface of B cells in lymphoid tissues. This antibody is useful for the identification of immunoglobulins in leukemias, plasmacytomas and some non-Hodgkin's lymphomas. |
| Immunogen | BALB/C mice were injected with purified human IgG myeloma proteins covalently coupled to polyaminostyren microbeads. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | HP6054 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IGLV214 V1-4 |
|---|---|
| Target | lambda Light chain |
| Cellular Localization | Cytoplasmic |
Application
IHC Image using - Detection of Lambda Light Chain by IHC in Human Tonsil| Positive Control | Tonsil |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
