| Product Name | IGF1R antibody [33255.111] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [33255.111] to IGF1 ReceptorThis receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| Immunogen | recombinant human IGFIR Glu31-Asn932 of accession # P08069 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 33255.111 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| For ELISA: Use at a concentration of 2 - 2.5μg/ml. For Neut: Use at a concentration of 10 - 12μg/ml. Neutralizes ~50-75% of IGF-I receptor-mediated rhIGF-I biological activity in MCF7 cells. Will block cell surface IGF-I receptor mediated IGF-I and IGF-II bioactivities. For WB: Use at a concentration of 1 - 2 μg/ml under non reducing conditions. Predicted molecular weight: 155 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD221 antibody, IGF1 receptor antibody, IGF1 receptor antibody, IGF1A antibody, IGF1R antibody, IGFI antibody, IGFI antibody, IGFIR antibody, IGFIRC antibody, IGFR antibody, Insulin like growth factor1 receptor antibody, Insulin like growth fact |
|---|---|
| Target | IGF1 receptor |
| Tissue Specificity | Does not cross-react with human recombinant IGFI bp1, human recombinant IGFI and human recombinant IGFII. |
| Cellular Localization | Plasma membrane |
| Application Notes | For ELISA: Use at a concentration of 2 - 2.5μg/ml. For Neut: Use at a concentration of 10 - 12μg/ml. Neutralizes ~50-75% of IGF-I receptor-mediated rhIGF-I biological activity in MCF7 cells. Will block cell surface IGF-I receptor mediated IGF-I and IGF-II bioactivities. For WB: Use at a concentration of 1 - 2 μg/ml under non reducing conditions. Predicted molecular weight: 155 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Prior to reconstitution store at 4°C. Following reconstitution keep as concentrated solution, aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | From PBS, 20 mM ammonium bicarbonate, =5% trehalose, 100 µg BSA Reconstitute with sterile PBS to the desired concentration. |
