Application
Serial dilutions of ELISA detection IL-1α recombinant protein antibody ranging from 500 to 4 pg/ml were used to obtain a linear standard curve| Product Name | IL1 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [364-3B3-14] to IL1 alphaInterleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence. |
| Immunogen | Recombinant human IL-1alpha |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 364-3B3-14 |
| Host Species | Mouse |
| Tested Applications | ELISA |
| ELISA: Use at a concentration of 2 - 6 μg/ml. To obtain a linear standard curve, serial dilutions of IL-1 alpha recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. ELISPOT: Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. This antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1 alpha -producing cells within mixed cell populations. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | Preinterleukin1 alpha Interleukin 1 alpha precursor ilif1 ilia Prointerleukin 1 alpha IL1 alpha Hematopoietin1 Hematopoietin 1 Interleukin1 alpha precursor Preinterleukin 1 alpha IL1A Prointerleukin1 alpha |
|---|---|
| Target | IL1 alpha |
| Cellular Localization | Secreted |
| Light Chain Type | kappa |
Application
Serial dilutions of ELISA detection IL-1α recombinant protein antibody ranging from 500 to 4 pg/ml were used to obtain a linear standard curve| Application Notes | ELISA: Use at a concentration of 2 - 6 μg/ml. To obtain a linear standard curve, serial dilutions of IL-1 alpha recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. ELISPOT: Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. This antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1 alpha -producing cells within mixed cell populations. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: PBS. pH 7.2 |
