Application
IHC analysis of colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue using IL1 beta antibody at a concentration of 0.25 μg/ml. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.| Product Name | IL1 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IL1 beta |
| Immunogen | Recombinant Rat IL-1β |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCNeutWB |
| We recommend the following starting dilutions: For WB: Use at a concentration of 0.1- 0.2 µg/ml. For IHC: Use at a concentration of 0.25 μg/ml. For ELISA: Use at a concentration of 0.5- 2.0 μg/ml. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Rat IL-1β (0.20 ng/ml), a concentration of 0.09- 0.14 µg/ml of this antibody is required.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Gene Synonyms | interleukin 1 beta Il1b |
|---|---|
| Target | IL1 beta |
Application
IHC analysis of colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue using IL1 beta antibody at a concentration of 0.25 μg/ml. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.| Application Notes | We recommend the following starting dilutions: For WB: Use at a concentration of 0.1- 0.2 µg/ml. For IHC: Use at a concentration of 0.25 μg/ml. For ELISA: Use at a concentration of 0.5- 2.0 μg/ml. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Rat IL-1β (0.20 ng/ml), a concentration of 0.09- 0.14 µg/ml of this antibody is required.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.2 |
