Application
IL10 antibody [GT5111] detects IL10 protein by western blot analysis. Non-transfected (-) and IL-10-transfected (+, including 3x Flag-tag) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody [GT5111] at a dilution of 1:5000.| Product Name | IL10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT5111] to IL10 (interleukin 10)The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IL10. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT5111 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rat, Dog, Cat, Rabbit, Sheep, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigRat (80%), Dog (80%), Cat (86%), Rabbit (83%), Sheep (85%), Rhesus Monkey (93%), Chimpanzee (98%), Bovine (81%), Guinea pig (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GVHDS IL10A TGIF IL-10 interleukin 10 CSIF |
|---|---|
| Target | IL10 |
| Molecular Weight(MW) | 21 kDa (note) |
| Cellular Localization | Secreted |
Application
IL10 antibody [GT5111] detects IL10 protein by western blot analysis. Non-transfected (-) and IL-10-transfected (+, including 3x Flag-tag) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody [GT5111] at a dilution of 1:5000.| Positive Control | 3x Flag human IL-10-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
