| Product Name | IL15 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [34593.11] to IL15IL15 (114 amino acids) has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha-helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle. |
| Immunogen | Recombinant full length human IL15 protein expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 34593.11 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| ELISA: Use at a concentration of 0.5-1 µg/ml. The detection limit for recombinant human IL15 is approximately 0.3 ng/well. Neut: Use at a concentration of 0.05-0.1 µg/ml in the presence of 10 ng/ml of recombinant human IL15 using the MO7e cell line. WB: Use at a concentration of 1-2 µg/ml. Predicted molecular weight: 12.5 kDa. The detection limit for recombinant human IL15 is approximately 20 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. Endotoxin level is: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | IL-15 MGC9721 IL 15 IL15 |
|---|---|
| Target | IL15 |
| Cellular Localization | Secreted |
| Positive Control | GTX10784 has the ability to neutralize the bioactivity of human IL15 on the human megakaryocytic leukemic cell line, MO7e |
|---|---|
| Application Notes | ELISA: Use at a concentration of 0.5-1 µg/ml. The detection limit for recombinant human IL15 is approximately 0.3 ng/well. Neut: Use at a concentration of 0.05-0.1 µg/ml in the presence of 10 ng/ml of recombinant human IL15 using the MO7e cell line. WB: Use at a concentration of 1-2 µg/ml. Predicted molecular weight: 12.5 kDa. The detection limit for recombinant human IL15 is approximately 20 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. Endotoxin level is: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Constituents: PBS |
