Application
Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-32 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | IL32 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT2F9] to IL-32The gene encoding IL32 is located on human chromosome 16p13.3 and is organized into eight exons. IL32 is mainly expressed in natural killer cells, T cells, epithelial cells, and blood monocytes. It can induce the proinflammatory cytokines TNF? and IL1? in murine peritoneal macrophages as well as in phorbol ester-differentiated human THP1 cells. In the inflamed lesions of chronic pancreas, the ductal expression of IL32 was markedly increased. Pancreatic duct cells are the local source of IL32, and IL32 may play an important role in inflammatory responses and pancreatic cancer growth. Recently, a synergism between IL32 and other well characterized players in innate immunity has been documented. |
| Immunogen | Recombinant human IL32 (1-131aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | AT2F9 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Interleukin32 isoform A IL32 TAIFd Interleukin 32 Tumor necrosis factor alphainducing factor (TAIF) IL 32delta TAIFc TAIF IL-32 IL32delta Interleukin32 NK 4 Interleukin 32 isoform A IL 32alpha TAIFb Natural killer cells protein4(NK4) IL 32ga |
|---|---|
| Target | IL-32 |
| Light Chain Type | kappa |
Application
Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-32 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide |
