Application
Structure of the recombinant antibody anti-IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a mouse IgG2b Fc region.Application
Binding of IL-33 (mouse) to ST2/IL-1RAcP is inhibited by IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1).Methods: IL-33 (mouse) was coated on an ELISA plate at 1μg/ml. IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 100μl of supernatant of cells containing ST2 (human):Fc/IL-1RAcP (human):Fc. After incubation for 1h at RT, the binding was detected using an anti-Fc human antibody (HRP).| Product Name | IL33 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [Bondy-1-1] to IL33 |
| Immunogen | Mouse recombinant IL-33. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | Bondy-1-1 |
| Host Species | Mouse |
| Tested Applications | ELISAFunctional Assay |
| Functional Assay: Inhibits the binding of mouse IL-33 to ST2/IL-1RAcP.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NF HEV interleukin 33 9230117N10Rik Il1f11 Il33 |
|---|---|
| Target | IL33 |
| Tissue Specificity | Recognizes mouse IL-33. |
Application
Structure of the recombinant antibody anti-IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a mouse IgG2b Fc region.
Application
Binding of IL-33 (mouse) to ST2/IL-1RAcP is inhibited by IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1).Methods: IL-33 (mouse) was coated on an ELISA plate at 1μg/ml. IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 100μl of supernatant of cells containing ST2 (human):Fc/IL-1RAcP (human):Fc. After incubation for 1h at RT, the binding was detected using an anti-Fc human antibody (HRP).| Application Notes | Functional Assay: Inhibits the binding of mouse IL-33 to ST2/IL-1RAcP.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20°C. |
| Storage Buffer | PBS containing 10% glycerol and 0.02% sodium azide. |
