Application
WB analysis of extracts from various cells using Importin alpha 2 antibody.Application
IHC-P analysis of human kidney using Importin alpha 2 antibody at a dilution of 1:200.Application
IHC-P analysis of human lung using Importin alpha 2 antibody at a dilution of 1:200.Application
IHC-P analysis of human stomach cancer using Importin alpha 2 antibody at a dilution of 1:200.Application
IHC-P analysis of rat brain using Importin alpha 2 antibody at a dilution of 1:200.Application
ICC/IF analysis of MCF7 cells using Importin alpha 2 antibody (green) and DAPI (blue).Application
ICC/IF analysis of A549 cells using Importin alpha 2 antibody (green) and DAPI (blue).| Product Name | Importin alpha 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Importin alpha 2The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human KPNA2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KPNA2 karyopherin alpha 2 (RAG cohort 1 importin alpha 1) RCH1 SRP1alpha IPOA1 QIP2 |
|---|---|
| Target | Importin alpha 2 |
| Molecular Weight(MW) | 58 kDa (note) |
Application
WB analysis of extracts from various cells using Importin alpha 2 antibody.
Application
IHC-P analysis of human kidney using Importin alpha 2 antibody at a dilution of 1:200.
Application
IHC-P analysis of human lung using Importin alpha 2 antibody at a dilution of 1:200.
Application
IHC-P analysis of human stomach cancer using Importin alpha 2 antibody at a dilution of 1:200.
Application
IHC-P analysis of rat brain using Importin alpha 2 antibody at a dilution of 1:200.
Application
ICC/IF analysis of MCF7 cells using Importin alpha 2 antibody (green) and DAPI (blue).
Application
ICC/IF analysis of A549 cells using Importin alpha 2 antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
