Application
Extracts of Integrin β1-deficient fibroblasts expressing wild type (1-5,7) or serine-785-alanine mutant (S785A: 6, 8) chicken Integrin β1 were resolved on a 10% tris-glycine gel and transferred to PVDF.| Product Name | Integrin beta 1 / CD29 (phospho Ser785) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Integrin beta 1 ( phospho S785 )Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell’s surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. Phosphorylation of serine 785 on the Integrin beta 1 promotes cell attachment, but inhibits spreading and migration, whereas dephosphorylation promotes cell spreading and migration. |
| Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Integrin beta 1. This sequence is conserved among many species including mouse, rat and chicken. |
| Modification | p-Ser785 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 µg/ml. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD29 FNRB GPIIA MDF2 MSK12 VLA-BETA VLAB VLA BETA VLABETA Integrin beta 1 (phospho S785) Integrin beta 1 (phospho Ser785) Integrin beta 1 (pS785) Integrin beta 1 (pSer785) Integrin beta 1 phospho S785 Integrin beta 1 phospho Ser785 Phospho I |
|---|---|
| Target | Integrin beta 1 |
| Tissue Specificity | Integrin beta 2 (55% identity) and Integrin beta 7 (64% identity) have also not been tested, but are not expected to react. |
Application
Extracts of Integrin β1-deficient fibroblasts expressing wild type (1-5,7) or serine-785-alanine mutant (S785A: 6, 8) chicken Integrin β1 were resolved on a 10% tris-glycine gel and transferred to PVDF.| Positive Control | Fibroblasts lacking Integrin beta 1 transfected with chicken Integrin beta 1 |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 µg/ml. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
