Application
Extracts of serum-starved mitotic HeLa cells generated by treatment with 100 ng/mL taxol for 16 hours were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.| Product Name | Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Integrin beta 1 ( phospho T788 + T789 )Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cellis surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. Phosphorylation of threonines 788 and 789 on the Integrin beta 1 receptor may play a key role in cell-cycle dependent regulation. |
| Immunogen | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Integrin beta 1 receptor that contains threonines 788 and 789 (based on Swiss Protein database, accession number P05558). The sequence is conserved |
| Modification | p-Thr788/Thr789 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD29 FNRB GPIIA MDF2 MSK12 VLA-BETA VLAB VLA BETA VLABETA Integrin beta 1 (phospho T788/T789) Integrin beta 1 (phospho Thr788/Thr789) Integrin beta 1 (pT788/pT789) Integrin beta 1 (pThr788/pThr789) Integrin beta 1 phospho T788/T789 Integrin b |
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| Target | Integrin beta 1 |
Application
Extracts of serum-starved mitotic HeLa cells generated by treatment with 100 ng/mL taxol for 16 hours were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.| Positive Control | HeLa cells in mitosis - Positive and negative controls available see GTX25215 |
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| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 130 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
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| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.3 containing 50% glycerol, 1mg/ml of BSA (IgG, protease free) and 0.05% sodium azide |
