| Product Name | Integrin beta 1 / CD29 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4B7] to Integrin beta 1Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. |
| Immunogen | Human ocular melanoma cell line V + B2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4B7 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-PIP |
| FACS: Use at an assay dependent dilution. IF: 1/125 - 1/250. IHC-P: 1/125 - 1/250. IP: Use at 2μg/mg of lysate. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | GPIIA fnrb vlabeta vlab Integrin beta 1 fibrinogen receptor beta subunit integrin vla4 beta subunit mdf2 cd29 Integrin beta1 msk12 itgb1 cd29 antigen very late activation protein beta polypeptide VLA-BETA VLA BETA |
|---|---|
| Target | Integrin beta 1 |
| Tissue Specificity | Specific for integrin beta-1 subunit or CD29, which runs at 110 kDa non-reduced and at 130 kDa reduced. |
| Cellular Localization | Type 1 membrane protein |
| Light Chain Type | kappa |
| Positive Control | Human spleen, thymus or tonsil |
|---|---|
| Application Notes | FACS: Use at an assay dependent dilution. IF: 1/125 - 1/250. IHC-P: 1/125 - 1/250. IP: Use at 2μg/mg of lysate. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline |
