Application
Western Blot analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL| Product Name | Integrin beta 1 / CD29 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 101A] to Integrin beta 1Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on the cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. |
| Immunogen | Tissue / cell preparation (Human). (Burkitts lymphoma cell line Raji). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-101A |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| FACS: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Integrin beta 1 Integrin beta1 CD29 FNRB GPIIA MDF2 MSK12 VLA-BETA VLAB VLA BETA VLABETA |
|---|---|
| Target | Integrin beta 1 |
Application
Western Blot analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Lane 1: lysate of human PBL Lane 2: lysate of canine PBL Lane 3: lysate of porcine PBL| Application Notes | FACS: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
