| Product Name | Integrin beta 1 / CD29 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4B7R] to Integrin beta 1CD29 (integrin beta-1 / gpIIa) is a cell adhesion molecule appearing on platelets, as the common beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. The CD29 is expressed at the cell surface exclusively as part of a heterodimer, in association with one of at least nine different integrin alpha subunits (alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6, alpha 7, alpha 8, and alpha v). With the exception of red blood cells and possible weak expression on granulocytes, CD29 is expressed on nearly all cell and tissue types. |
| Immunogen | Human melanoma V+B2 cell line. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4B7R |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-P |
| FACS: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 10 - 20 μg/ml for 60 min at RT.. Perform enzymatic antigen retrieval before commencing with IHC staining protocol (protease XXV at 1mg/ml PBS for 5 minutes at 37°C or digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanPig |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Integrin beta 1 Integrin beta1 CD29 FNRB GPIIA MDF2 MSK12 VLA-BETA VLAB VLA BETA VLABETA |
|---|---|
| Target | Integrin beta 1 |
| Cellular Localization | Cell Membrane |
| Light Chain Type | kappa |
| Positive Control | Tonsil |
|---|---|
| Application Notes | FACS: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 10 - 20 μg/ml for 60 min at RT.. Perform enzymatic antigen retrieval before commencing with IHC staining protocol (protease XXV at 1mg/ml PBS for 5 minutes at 37°C or digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: 0.2% BSA, 10mM PBS. pH 7.4 |
