Application
Fluorescent confocal image of U251 cell stained with IPF Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IPF primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). IPF immunoreactivity is localized to Cytoplasm and Nucleus significantly.Application
Ipf Antibody (C-term) western blot analysis in U-937 cell line lysates (35ug/lane).This demonstrates the Ipf antibody detected the Ipf protein (arrow).| Product Name | IPF antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IPF (C-term)The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 193~223 amino acids from the C-terminal region of human IPF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Glucosesensitive factor IPF1 Islet/duodenum homeobox-1 IUF1 Insulin upstream factor 1 IDX1 Pancreas/duodenum hom Insulin upstream factor1 STF-1 IUF-1 Islet/duodenum homeobox1 Somatostatintransactivating factor 1 IDX-1 Insulin promoter factor1 |
|---|---|
| Target | IPF |
| Molecular Weight(MW) | 30.771 kDa (note) |
Application
Fluorescent confocal image of U251 cell stained with IPF Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IPF primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). IPF immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Application
Ipf Antibody (C-term) western blot analysis in U-937 cell line lysates (35ug/lane).This demonstrates the Ipf antibody detected the Ipf protein (arrow).| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
