Application
Extracts of 293T cells transfected with wild-type human IRS-1 and treated with 100ng/mL TPA for 30 minutes were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.| Product Name | IRS1 (phospho Ser616) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IRS1 ( phospho S616 )Insulin Receptor Substrate 1 (IRS 1) is a 165 kDa multi-phosphorylated cytoplasmic docking protein involved in metabolic and proliferative signaling by insulin, IL-4, and other cytokines. IRS 1 functions to enhance growth hormone-induced proliferative signaling. Activation of protein kinase C stimulates a kinase which phosphorylates IRS 1 at serine 616, resulting in inhibition of insulin signaling in the cell, possibly serving as a potential mechanism for insulin resistance in some forms of obesity. |
| Immunogen | Synthetic peptide derived from a region of IRS 1 that contains serine 616. The sequence is conserved in human, mouse, and rat. |
| Modification | p-Ser616 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 165 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIRS-1 HIRS 1 HIRS1 IRS1 (phospho S616) IRS1 (phospho Ser616) IRS1 (pS616) IRS1 (pSer616) IRS1 phospho S616 IRS1 phospho Ser616 Phospho IRS1 (S616) Phospho IRS1 (Ser616) Phospho IRS1 (pS616) Phospho IRS1 (pSer616) |
|---|---|
| Target | IRS1 |
Application
Extracts of 293T cells transfected with wild-type human IRS-1 and treated with 100ng/mL TPA for 30 minutes were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.| Positive Control | CHO cell line transfected with a vector encoding the human insulin receptor (CHO-T) and transiently transfected with a vector encoding IRS 1, stimulated with a phorbol ester, TPA |
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| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 165 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 1.0mg/ml BSA (IgG, protease free). pH 7.4 |
