Application
The data show that only the phosphopeptide corresponding to IRS-1 [pY1179] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show up-regulation of IRS-1 [pY1179] upon treatment with insulin in this cell system.| Product Name | IRS1 (phospho Tyr1179) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IRS1 ( phospho Y1179 )Insulin Receptor Substrate 1 (IRS 1) is a 165 kDa multi-phosphorylated cytoplasmic docking protein involved in the regulation of metabolism and proliferation by insulin, IL-4, and other cytokines. Insulin binding to the insulin receptor activates the catalytic domain and causes phosphorylation on tyrosine residues of IRS proteins, which in turn serve to recruit a variety of Src homology 2 (SH2) domain-containing proteins such as PI3 kinase, Grb 2, SHP 2, Nck and Crk. These proteins further propagate intracellular signalling, culminating in both metabolic and growth-promoting functions of IRS 1. pTyr1179 of IRS 1 is a SHP 2 binding site, which serves to negatively regulate insulin signalling. |
| Immunogen | Synthetic peptide (Mouse) derived from a region of mouse IRS 1 that contains tyrosine 1173 (corresponding to tyrosine 1179 of the human sequence). |
| Modification | p-Tyr1179 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 165 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIRS-1 HIRS 1 HIRS1 IRS1 (phospho Y1179) IRS1 (phospho Tyr1179) IRS1 (pY1179) IRS1 (pTyr1179) IRS1 phospho Y1179 IRS1 phospho Tyr1179 Phospho IRS1 (Y1179) Phospho IRS1 (Tyr1179) Phospho IRS1 (pY1179) Phospho IRS1 (pTyr1179) |
|---|---|
| Target | IRS1 |
| Tissue Specificity | IRS 3 (50% homologous) also may react, but has not been tested. |
Application
The data show that only the phosphopeptide corresponding to IRS-1 [pY1179] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show up-regulation of IRS-1 [pY1179] upon treatment with insulin in this cell system.| Positive Control | Chinese Hamster Ovary cell line transfected with a vector encoding the human insulin receptor (CHO-T) and transiently transfected with a vector encoding IRS 1 and stimulated with insulin |
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| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 165 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA (IgG, protease free). pH 7.4 |
