Application
IRS-1 [pY612] PAb Western blot| Product Name | IRS1 (phospho Tyr612) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IRS1 ( phospho Y612 )Insulin Receptor Substrate 1 (IRS 1) is a 165 kDa multi-phosphorylated cytoplasmic docking protein involved in the regulation of metabolism and proliferation by insulin, IL-4, and other cytokines. Insulin binding to the insulin receptor activates the catalytic domain and causes phosphorylation on tyrosine residues of IRS proteins, which in turn serve to recruit a variety of Src homology 2 (SH2) domain-containing proteins such as PI3 kinase, Grb 2, SHP 2, Nck and Crk. These proteins further propagate intracellular signalling, culminating in both metabolic and growth-promoting functions of IRS 1. pTyr612 of IRS 1 is in a pYMxM consensus motif PI3 kinase binding site. |
| Immunogen | Synthetic peptide derived from a region of IRS 1 that contains tyrosine 612. The sequence is conserved in human, mouse, rat, and chicken. |
| Modification | p-Tyr612 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 165 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIRS-1 HIRS 1 HIRS1 IRS1 (phospho Y612) IRS1 (phospho Tyr612) IRS1 (pY612) IRS1 (pTyr612) IRS1 phospho Y612 IRS1 phospho Tyr612 Phospho IRS1 (Y612) Phospho IRS1 (Tyr612) Phospho IRS1 (pY612) Phospho IRS1 (pTyr612) |
|---|---|
| Target | IRS1 |
| Tissue Specificity | IRS 2 (85% homologous) has also not been tested. |
Application
IRS-1 [pY612] PAb Western blot| Positive Control | Chinese Hamster Ovary cell line transfected with a vector encoding the human insulin receptor (CHO-T) and transiently transfected with a vector encoding IRS 1 and stimulated with insulin |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Predicted molecular weight: 165 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA (IgG, protease free). pH 7.4 |
