Application
Cell lysates of HeLa(each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system| Product Name | ISG15 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3E5 ] to ISG15ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play important roles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15 is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety of proteins when cells are treated with type I interferon or lipopolysaccharide. |
| Immunogen | Recombinant human ISG15 (1-157aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3E5 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:1,000 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ISG15 Interferon induced17 kDa protein GIP2 Ubiquitin c Interferon induced 17 kDa protein G1P2 Interferon alpha inducible protein 15 kDa ubiquitin-like modifier IFI 15 ISG 15 Interferon stimulated protein15 kDa Interferon stimulated protein 15 k |
|---|---|
| Target | ISG15 |
| Cellular Localization | UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted. |
| Light Chain Type | kappa |
Application
Cell lysates of HeLa(each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system| Application Notes | Western Blot: Use at 1:1,000 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
