Application
Jab1 antibody detects Jab1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Jab1 antibody diluted at a dilution of 1:500.Application
ApplicationApplication
Sample (50 ug of whole cell lysate) A: Rat liver 10% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000| Product Name | Jab1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human Jab1JAB-1 is a 38 kDa protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the ""Signalosome"", which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants, which is important in light-mediated plant development. Although the specific function of JAB-1 in this complex is unclear, it has recently been implicated in targeting proteins for degradation by the proteasome. |
| Immunogen | The full-length mouse JAB-1 gene expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2A10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| For IP: Use at a concentration of 0.5 - 1 µg/ml. For WB: Use at a dilution of 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseDrosophilaRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Jab 1 Jab-1 Jab1 JabI |
|---|---|
| Target | Jab1 |
| Molecular Weight(MW) | 38 kDa (note) |
| Cellular Localization | Nuclear/Cytoplasmic |
| Light Chain Type | kappa |
Application
Jab1 antibody detects Jab1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Jab1 antibody diluted at a dilution of 1:500.
Application
Application
Application
Sample (50 ug of whole cell lysate) A: Rat liver 10% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000| Positive Control | 293T , A431 , HeLa , HepG2 , MCF-7 , MDA-MB-231 , Rat liver |
|---|---|
| Application Notes | For IP: Use at a concentration of 0.5 - 1 µg/ml. For WB: Use at a dilution of 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
