Application
Customer Review using - D10 T cells were lysed in IP buffer (20mM Tris-HCl, pH 7.5, 50mM NaCl, 50mM NaF, 10mM -glycerophosphate, 1% Triton X-100, 1mM PMSF).Application
Part of a customer review usingApplication
Detection of human Jab1 protein in HeLa whole cell lysate using GeneTex monoclonal antibody| Product Name | Jab1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human Jab1JAB-1 is a 38a protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the ""Signalosome"", which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants, which is important in light-mediated plant development. Although the specific function of JAB-1 in this complex is unclear, it has recently been implicated in targeting proteins for degradation by the proteasome. |
| Immunogen | The full-length mouse JAB-1 gene expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 8H8.5 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseDrosophila |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Jab 1 Jab-1 Jab1 JabI |
|---|---|
| Target | Jab1 |
| Molecular Weight(MW) | 38 kDa (note) |
| Cellular Localization | Nuclear/Cytoplasmic |
| Light Chain Type | kappa |
Application
Customer Review using - D10 T cells were lysed in IP buffer (20mM Tris-HCl, pH 7.5, 50mM NaCl, 50mM NaF, 10mM -glycerophosphate, 1% Triton X-100, 1mM PMSF).
Application
Part of a customer review using
Application
Detection of human Jab1 protein in HeLa whole cell lysate using GeneTex monoclonal antibody| Positive Control | 293 cell lysate |
|---|---|
| Application Notes | Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
