Application
WB ImageWestern blot using| Product Name | JNK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to JNK1MAP kinases consist of several subgroups, including the ERK, JNK, and p38 kinases. The members are regulated by many different extracellular cues ranging from cytokines, growth factors, and neuropeptides. These stimuli activate cell surface receptors to stresses such as cold, heat, osmolarity changes and irradiation. The pathways regulated by the MAPKs control a broad array of cellular responses ranging from survival, cell proliferation, and apoptosis. The MAPKs family is also characterized by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation. The closely related ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif. While JNK1 is activated by dual phosphorylation at a T-P-Y motif and p38 is also activated by dual phosphorylation at a T-G-Y motif. |
| Immunogen | Synthetic peptide (Human) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1 ug/ml Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | JNK JNK1 JNK1A2 JNK21B1/2 PRKM8 SAPK1 |
|---|---|
| Target | JNK1 |
| Tissue Specificity | This antibody does not detect JNK2 or JNK3. |
Application
WB ImageWestern blot using| Positive Control | PC12 cell lysate |
|---|---|
| Application Notes | WB: 1 ug/ml Optimal dilution / concentration should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline (without Mg2+ and Ca2+) containing 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide |
