Application
Immunofluorescence staining of methanol-fixed A431(left) and Hela(right) cells showing cytoplasmic and membrane localization.Application
Western blot analysis using anti-MAPK10 mAb against NIH/3T3 (1) and SKN-SH (2) cell lysate.| Product Name | JNK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [10E4A4] to JNK3The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 28 and 233 of human MAPK10 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10E4A4 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | JNK3 SAPK1B JNK3A P493F12 P54BSAPK PRKM10 |
|---|---|
| Target | JNK3 |
| Molecular Weight(MW) | 53 kDa (note) |
Application
Immunofluorescence staining of methanol-fixed A431(left) and Hela(right) cells showing cytoplasmic and membrane localization.
Application
Western blot analysis using anti-MAPK10 mAb against NIH/3T3 (1) and SKN-SH (2) cell lysate.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
