Application
Detection of Ki67 by IHC in Human TonsilApplication
Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody| Product Name | Ki67 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Polyclonal Antibody to Human Ki - 67 AntigenThis antibody reacts with a nuclear antigen present in proliferating human cells. This antibody cross-reacts with a range of mammalian species. It can be used to evaluate the ki-67 labeling index in various tumors. |
| Immunogen | Synthetic peptide from 62 base pair region of the human Ki-67 antigen. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Not tested | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIA |
|---|---|
| Target | Ki67 |
| Tissue Specificity | This antibody reacts with a nuclear antigen present in proliferating human cells. |
| Cellular Localization | Cytoplasmic |
Application
Detection of Ki67 by IHC in Human Tonsil
Application
Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Not tested |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS with 1% BSA and 0.05% sodium azide in it. |
