Application
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using microwave-treated human tonsil sections| Product Name | Ki67 antibody [PP-67] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [PP - 67] to Ki67 - Proliferation MarkerKi-67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. The monoclonal antibody is useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labeling index. The correlation between low Ki67 index and histologically low-grade tumors is strong. |
| Immunogen | A HeLa cell nuclear preparation. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | PP-67 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-PWB |
| For IHC-Fr: Use at an assay dependent dilution. For IHC-P: Use at a dilution of 1:800. Perform heat mediated antigen retrieval using microwave method. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIA |
|---|---|
| Target | Ki67 |
Application
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using microwave-treated human tonsil sections| Application Notes | For IHC-Fr: Use at an assay dependent dilution. For IHC-P: Use at a dilution of 1:800. Perform heat mediated antigen retrieval using microwave method. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Contains 15 mM sodium azide |
