| Product Name | Ku70 + Ku80 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [162] to Ku70 + Ku80The Ku autoantigen is a heterodimer of 70 kDa (p70) and ~80 kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. |
| Immunogen | Human B cell nuclei from plasmacytoid 2p68 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 162 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-PIPWBRIP |
| Flow Cytometry / Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 2μg/mg protein lysate. Immunohistology (formalin fixed paraffin embedded): use at a concentration 1 - 2μg/ml for 30 min at RT (antigen retrieval is not required). Optimal dilutions / concentrations should be determined by the end user. This antibody is not suitable for Western blot.: |
|
| Species Reactivity | HumanMouseMonkeyRatXenopus |
| Concentration | 0.2mg/ml |
| Gene Synonyms | CTC75 FLJ39089 CTCBF KARP-1 G22P1 KARP1 KU70 KU80 ML8 KUB2 TLAA Ku86 NFIV KARP 1 |
|---|---|
| Target | Ku70 + Ku80 |
| Cellular Localization | Nuclear. |
| Positive Control | HeLa cells. Tonsil. |
|---|---|
| Application Notes | Flow Cytometry / Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 2μg/mg protein lysate. Immunohistology (formalin fixed paraffin embedded): use at a concentration 1 - 2μg/ml for 30 min at RT (antigen retrieval is not required). Optimal dilutions / concentrations should be determined by the end user. This antibody is not suitable for Western blot.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
