| Product Name | Ku80 antibody [111] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [111] to Ku80The Ku autoantigen is a heterodimer of 70 kDa (p70) and ~80 kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. |
| Immunogen | Human B cell nuclei from plasmacytoid 2p68 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 111 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIPWB |
| For FACS: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. For IP (native and denatured) Use protein G at a concentration of 2 μg/mg protein lysate. For WB: Use at a concentration of 0.5-1.0 μg/mg protein lysate. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMonkey |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | FLJ39089, KARP-1, KARP1, KU80, KUB2, Ku86, NFIV, KARP 1 |
|---|---|
| Target | Ku80 |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
| Positive Control | HeLa or BT474 cells |
|---|---|
| Application Notes | For FACS: Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. For IP (native and denatured) Use protein G at a concentration of 2 μg/mg protein lysate. For WB: Use at a concentration of 0.5-1.0 μg/mg protein lysate. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM Phosphate-buffered saline, pH7.4 containing 0.2% BSA and 0.09% sodium azide |
