Application
Detection of human Ku80 protein in HeLa whole cell lysate using GeneTex Ku80 149.8 monoclonal antibodyApplication
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE diluted at 1:10000Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE diluted at 1:10000Application
Ku80 antibody [149.8] detects Ku80 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ku80 protein stained by Ku80 antibody [149.8] diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | Ku80 antibody [149.8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human Ku80Originally identified as an autoantigen, the Ku protein are nuclear proteins found in eukaryotes. They exist as a heterodimer consisiting of Ku 70 and Ku 80, migrating at approximately 70 and 80 kDa respectively. They bind to DNA ends at double strand breaks. The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK) and participate in the targeting of DNA-PK to DNA. The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signalling, cell proliferation, transcriptional activation and apoptosis. |
| Immunogen | Recombinant antigen containing the carboxy terminal portion of human Ku80 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 149.8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ39089, KARP-1, KARP1, KU80, KUB2, Ku86, NFIV, KARP 1 |
|---|---|
| Target | Ku80 |
Application
Detection of human Ku80 protein in HeLa whole cell lysate using GeneTex Ku80 149.8 monoclonal antibody
Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE diluted at 1:10000
Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE diluted at 1:10000
Application
Ku80 antibody [149.8] detects Ku80 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ku80 protein stained by Ku80 antibody [149.8] diluted at 1:500. Blue: Hoechst 33343 staining.| Positive Control | Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG |
|---|---|
| Application Notes | Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
