Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug D: 293T 1ug 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using Ku80 antibody (green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2000.Application
at a 1/2000 dilution staining ~ 80 kDa Ku80 in 35mg of T24 whole cell extract. ECL detection.| Product Name | Ku80 antibody [9403] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human Ku80Originally identified as an autoantigen, the Ku protein are nuclear proteins found in eukaryotes. They exist as a heterodimer consisiting of Ku 70 and Ku 80, migrating at approximately 70 and 80 kDa respectively. They bind to DNA ends at double strand breaks. The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK) and participate in the targeting of DNA-PK to DNA. The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signalling, cell proliferation, transcriptional activation and apoptosis. |
| Immunogen | Recombinant antigen containing the amino terminal portion of human Ku80 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 9403 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:1000-1:5000. For ICC/IF: Use at a dilution of 1:1000. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ39089, KARP-1, KARP1, KU80, KUB2, Ku86, NFIV, KARP 1 |
|---|---|
| Target | Ku80 |
| Molecular Weight(MW) | 80 kDa (note) |
| Cellular Localization | Nuclear |
Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug D: 293T 1ug 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Ku80 antibody (green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2000.
Application
at a 1/2000 dilution staining ~ 80 kDa Ku80 in 35mg of T24 whole cell extract. ECL detection.| Positive Control | T24 , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1000-1:5000. For ICC/IF: Use at a dilution of 1:1000. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
