Application
Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody [GT9712] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% methanol for 5 min. Green: Lamin A + C protein stained by Lamin A + C antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Lamin A + C antibody [GT9712] detects Lamin A + C protein by Western blot analysis. A. 30 μg HeLa whole cell lysate/extract B. 30 μg HeLa nuclear lysate/extract 7.5 % SDS-PAGE Lamin A + C antibody [GT9712] dilution: 1:1000Application
Immunoprecipitation of Lamin A + C protein from HeLa whole cell extracts using 5 μg of Lamin A + C antibody [GT9712] . Western blot analysis was performed using Lamin A + C antibody [GT9712] . EasyBlot anti-Mouse IgG was used as a secondary reagent.| Product Name | Lamin A + C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [GT9712] to Lamin A + CThe nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Lamin A/C. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT9712 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rhesus Monkey, ChimpanzeeMouse (84%), Rat (89%), Dog (96%), Pig (96%), Rhesus Monkey (99%), Chimpanzee (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDCD1 antibody CDDC antibody CMD1A antibody CMT2B1 antibody EMD2 antibody FPL antibody FPLD antibody HGPS antibody IDC antibody LDP1 antibody LFP antibody LGMD1B antibody LMN1 antibody LMNC antibody LMNL1 antibody PRO1 an |
|---|---|
| Target | lamin A/C |
| Molecular Weight(MW) | 65 KDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody [GT9712] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% methanol for 5 min. Green: Lamin A + C protein stained by Lamin A + C antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Lamin A + C antibody [GT9712] detects Lamin A + C protein by Western blot analysis. A. 30 μg HeLa whole cell lysate/extract B. 30 μg HeLa nuclear lysate/extract 7.5 % SDS-PAGE Lamin A + C antibody [GT9712] dilution: 1:1000
Application
Immunoprecipitation of Lamin A + C protein from HeLa whole cell extracts using 5 μg of Lamin A + C antibody [GT9712] . Western blot analysis was performed using Lamin A + C antibody [GT9712] . EasyBlot anti-Mouse IgG was used as a secondary reagent.| Positive Control | HeLa , HeLa nuclear |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
