Application
Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-human LAT (surface markers were stained prior to intracellular staining of human LAT). Panel A: gate on CD4+ cells Panel B: gate on CD8+ cells| Product Name | LAT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [LAT - 01] to LATLinker for activation of T cells (LAT) is an integral membrane protein (36 to 38 kD), which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. LAT antibody stains thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and spleen. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells. |
| Immunogen | Recombinant fragment (Human). (Entire cytoplasmic domain). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | LAT-01 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/500. Detects a band of approximately 36 - 38 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LAT1 pp36 |
|---|---|
| Target | LAT |
| Tissue Specificity | This antibody does not optimally recognize tyrosine-phosphorylated LAT. |
| Cellular Localization | Cell Membrane |
Application
Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-human LAT (surface markers were stained prior to intracellular staining of human LAT). Panel A: gate on CD4+ cells Panel B: gate on CD8+ cells| Application Notes | IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/500. Detects a band of approximately 36 - 38 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
Data sheet for OM261250