Application
Background extracts alone (1) or with added recombinant active human Lck protein (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, and then incubated with the Lck [pS192] antibody in a 1% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4) or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and signals were detected. The data show that only the phosphopeptide corresponding to Lck [pY192] blocks the antibody signal, demonstrating the specificity of the antibody.| Product Name | Lck (phospho Tyr192) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Lck ( phospho Y192 )Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of multiple sites, including the two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Phosphorylation of Lck Tyr-192 blocks high affinity binding of other proteins to the SH2 domain of Lck, thereby negatively regulating its participation in T cell antigen receptor signalling. |
| Immunogen | Synthetic peptide (Human) derived from the region of human Lck that contains tyrosine 192 (based on Swiss Protein database, accession number P06239). The sequence is conserved in human and mouse. |
| Modification | p-Tyr192 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 µg/ml. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LSK YT16 p56lck pp58lck Lck (phospho Y192) Lck (phospho Tyr192) Lck (pY192) Lck (pTyr192) Lck phospho Y192 Lck phospho Tyr192 Phospho Lck (Y192) Phospho Lck (Tyr192) Phospho Lck (pY192) Phospho Lck (pTyr192) |
|---|---|
| Target | Lck |
| Tissue Specificity | Lck (79% homologous), Hck (79% homologous) and Lyn (93% homologous) have not been tested. |
| Cellular Localization | Cytoplasmic |
Application
Background extracts alone (1) or with added recombinant active human Lck protein (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, and then incubated with the Lck [pS192] antibody in a 1% BSA-TBST buffer for two hours at room temperature, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4) or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and signals were detected. The data show that only the phosphopeptide corresponding to Lck [pY192] blocks the antibody signal, demonstrating the specificity of the antibody.| Positive Control | Full length untagged recombinant human Lck protein |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 µg/ml. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA (IgG, protease free). pH 7.4 |
