Application
Western Blot analysis (reducing conditions) of human Lck in whole cell lysate using anti-human Lck (LCK-01) . Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with Lck Lane 2: HEK-293T cell line transfected with Lck Lane 3: HEK-293T cell line (non-transfected)| Product Name | Lck antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [LCK - 01] to LckLck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is known to be regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). |
| Immunogen | Synthetic peptide, corresponding to amino acids 22-36 of Human Lck p56. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | LCK-01 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIPWB |
| FACS: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LSK YT16 p56lck pp58lck |
|---|---|
| Target | Lck |
| Tissue Specificity | The antibody recognizes defined epitope (aa 22-36) on p56 lck . |
| Cellular Localization | Cytoplasmic |
Application
Western Blot analysis (reducing conditions) of human Lck in whole cell lysate using anti-human Lck (LCK-01) . Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with Lck Lane 2: HEK-293T cell line transfected with Lck Lane 3: HEK-293T cell line (non-transfected)| Positive Control | Jurkat cell lysate |
|---|---|
| Application Notes | FACS: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
