| Product Name | Lck (phospho Tyr505) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Lck ( phospho Y505 )Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Tyr-505 is located near the carboxyl terminus of Lck and, when phosphorylated, associates intramolecularly with the SH2 domain in the amino-terminal half of the protein. This helps stabilise Lck in a conformation that, biologically, is relatively inactive. In the absence of phosphorylation at Tyr-505, intramolecular binding of the carboxyl terminus to the SH2 domain does not occur, and Lck exhibits increased activity in vivo. |
| Immunogen | Synthetic peptide (Human) derived from the region of Lck that contains tyrosine 505 (based on Swiss Protein database, accession number P06239). The sequence is conserved in human and mouse. |
| Modification | p-Tyr505 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 µg/ml. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LSK YT16 p56lck pp58lck Lck (phospho Y505) Lck (phospho Tyr505) Lck (pY505) Lck (pTyr505) Lck phospho Y505 Lck phospho Tyr505 Phospho Lck (Y505) Phospho Lck (Tyr505) Phospho Lck (pY505) Phospho Lck (pTyr505) |
|---|---|
| Target | Lck |
| Tissue Specificity | Hck [pY522] (75% homology), and Lyn [pY508] (83.3% homology) have not been tested. |
| Cellular Localization | Cytoplasmic |
| Positive Control | Full length untagged recombinant human Lck protein |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 µg/ml. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA (IgG, protease free). pH 7.4 |
